Reuse of treated urban wastewater on the growth and physiology of Medicago sativa L. cv. Gea and Petroselinum crispum L. cv. Commun: correlation with oxydative stress and DNA damage.

The freshwater scarcity is one of the major environmental problems, which is why the water reuse has become a possible remedy to cope with the shortage of water needed for agriculture irrigation. This study focuses on the evaluation of the irrigation effect with treated effluent from wastewater treatment plant in Tunisia on parsley (Petroselinum crispum L. cv. Commun) used as human food and alfalfa (Medicago sativa L. cv. Gea) as animal food. In vitro germination test was conducted at different dilution levels of wastewater as rejected into the environment (25, 50, and 100%) and wastewater with further treatment (TWW). Results have shown that wastewater with dilution of 25% as well as TWW positively affected the physiological parameters in comparison with the dilutions 50 and 100%. However, the tap water (TW) applied as control treatment has shown the best effects. Oxidative stress evaluated by malondialdehyde (MDA) content was in agreement with the physiological results and showed that the most stressed seeds were those treated with the dilutions 50 and 100%. A pot trial was also conducted to evaluate the suitability of WW and TWW in comparison to TW. Results have shown that TWW is more adapted than WW for irrigation as an improvement of growth and physiological parameters was recorded. Oxidative stress assessed with MDA and proline content has shown that plants irrigated with WW significantly accumulate MDA and proline compared to TWW. The TW has shown the lowest values. DNA damage was evaluated by extraction and agarose gel electrophoresis. It has revealed degradation of DNA for plants irrigated with WW. According to these results, it can be concluded that TWW can be used for irrigation of plants destined for human or animal foods. So, it can be a hydric alternative to resolve the problem of water deficit in semi-arid countries.

Occurrence and seasonal variation of plasticizers in sediments and biota from the coast of Mahdia, Tunisia.

Plasticizers are compounds often involved in the manufacturing of plastic products. Nevertheless, the ageing of the latter generates plasticizers that generally end up in the marine environment. In fact, marine pollution by phthalate acid esters (PAEs) and their alternatives has become an environmental and health issue of serious concern, as they are largely and ubiquitously present in the environment and aquatic organisms. In the present study, four PAEs, such as diethyl phthalate (DEP), diisobutyl phthalate (DiBP), dibutyl phthalate (DBP), and di(2-ethylhexyl) phthalate (DEHP), and one non-phthalate plasticizer (NPP), namely di-2-ethylhexyl terephthalate (DEHT), are wanted in different marine compartments from the coast of Mahdia in Tunisia such as sediment, seagrass, and mussel. The most abundant and frequently detected congener was DEHT at the concentrations reached 1.181 mg/kg in the sediment, 1.121 mg/kg in the seagrass, and 1.86 mg/kg in the mussel. This result indicates that the DEHT could emerge through the food chain and therefore bioaccumulate in marine compartments. In addition, we noticed that the seasonal variations of plasticizers were seriously affected by environmental factors including industrial and urban discharges.

Assessment of Bacterial Diversity of Industrial Poultry Wastewater by Denaturing Gradient Gel Electrophoresis (DGGE) and the Cultivation Method in Order to Inform Its Reuse in Agriculture.

Effluents discharged by poultry meat industries are heavily polluted with raw materials, such as fat, blood residues, and proteins. Thus, untreated effluents directly discharged into the environment may constitute a public health threat. This study aims to evaluate the bacterial diversity of three water qualities: industrial poultry wastewater (PWW), tap water (TW), and PWW diluted with TW (50 : 50) (V/V) (TWPWW) by the combination of culture-independent and culture-dependent approaches. The total bacterial DNA was extracted using phenol/chloroform method. The hypervariable 16S rRNA region V3-V5 was amplified by PCR using universal primers. The amplicons were separated by vertical electrophoresis on a polyacrylamide gel of increasing denaturing gradient according to their richness in GC bases. Selected bands were reamplified and sequenced. Pure isolated bacteria from nutrient agar medium were characterized according to their morphological and biochemical characteristics. Genomic DNA from pure strains was extracted by boiling method, and a molecular amplification of the 16S-23S ITS region of the 16S rRNA gene was performed using the universal primers. Selected isolates were identified by sequencing. Results showed a high bacterial load and diversity in PWW in comparison with TW and TWPWW. A collection of 44 strains was obtained, and 25 of them were identified by sequencing. Proteobacteria represented 76% of isolated bacteria Gamma-Proteobacteria was the predominate isolate (68%). Other isolates were Firmicutes (8%), Bacteroidetes (12%), and Actinobacteria (8%). These isolates belong to different genera, namely, Pseudomonas, Acinetobacter, Proteus, Empedobacter, Corynebacterium, Enterobacter, Comamonas, Frondibacter, Leclercia, Staphylococcus, Atlantibacter, Klebsiella, and Microbacterium.

Activity of Essential Oils Against Multidrug-Resistant Salmonella enteritidis.

We have investigated the antibacterial, anti-biofilm, and anti-quorum sensing potencies of six Essential Oils (EOs) obtained from cinnamon (Cinnamomum verum), thyme (Thymus vulgaris), clove (Eugenia caryophyllata), curcuma (Curcuma longa L.), rosemary (Rosmarinus officinalis L.), and sage (Salvia officinalis). The study was conducted on 20 multidrug-resistant (MDR) S. enteritidis clinical strains. Minimum inhibitory concentrations and minimum bactericide concentrations were displayed by microdilution. The effect on biofilm formation was tested on polystyrene plates. The anti-quorum sensing effect was determined by measuring the inhibition of violacein production by Chromobacterium violaceum CV026. The influence of EOs on the adhesion of Salmonella strains to HT-29 cells was studied. The potency of S. enteritidis to infect and kill Caenorhabditis elegans was evaluated. The cinnamon, thyme, and clove EOs showed remarkable antibacterial properties. Biofilm formation was significantly reduced by the six EOs: 99.10% for cinnamon, 97.64% for clove, 95.90% for thyme, 79.84% for rosemary, 28.98% for curcuma, and 15.55% for sage. The MIC/2 of clove EO exhibited the highest percentage of inhibition of violacein production (99.03%), followed by thyme (91.68%) and cinnamon (84.13%) EOs. Thyme extracts exhibited an important anti-adhesive potency. Clove EO behaves as an effective regulator of Salmonella virulence in nematodes.

A multi-biomarker approach for the early assessment of the toxicity of hospital wastewater using the freshwater organism Daphnia magna.

Hospital wastewater (HWW) contains different hazardous substances resulting from a combination of medical and non-medical activities of hospitals, including pharmaceutical residues. These substances may represent a threat to the aquatic environment if they do not follow specific treatment processes. Therefore, we aimed to investigate the effects of the untreated effluent collected from a general hospital in Mahdia City (Tunisia) on neonatal stages of the freshwater crustacean Daphnia magna. Test organisms were exposed to three proportions (3.12%, 6.25%, and 12.5% v/v) of HWW. After 48 h of exposure, a battery of biomarkers was measured, including the quantification of antioxidant enzymes [catalase (CAT) and total and selenium-dependent glutathione peroxidase (total GPx; Se-GPx)], phase II biotransformation isoenzymes glutathione-S-transferases (GSTs), cyclooxygenases (COX) involved in the regulation of the inflammatory process, and total cholinesterases (ChEs) activities. Lipid peroxidation (LPO) was measured to estimate oxidative damage. The here-obtained results showed significant decreases of CAT and GSTs activities and also on LPO content in daphnids, whereas Se-GPx activity was significantly increased in a dose-dependent manner. Impairment of cholinesterasic and COX activities were also observed, with a significant decrease of ChEs and an increase of COX enzymatic activities. Considering these findings, HWW was capable of inducing an imbalance of the antioxidant defense system, but without resulting in oxidative damage in test organisms, suggesting that peroxidases and alternative detoxifying pathways were able to prevent the oxidant potential of several drugs, which were found in the tested effluents. In general, this study demonstrated the toxicity of hospital effluents, measured in terms of the potential impairment of key pathways, namely neurotransmission, antioxidant defense, and inflammatory homeostasis of crustaceans.

In vivo toxicities of the hospital effluent in Mahdia Tunisia.

Hospital effluent (HE) is one of the most important sources of pharmaceuticals released into the environment. This kind of pollution is a recognized problem for both human health and aquatic life. Consequently, in the present study, we assessed the effects of untreated hospital effluent on mice via biochemical and histopathological determinations. Female mice were given free access to water bottles containing untreated HE at different dilutions for 21 days. Then clinical biochemistry and histopathology evaluation were conducted. Serum biochemistry analysis showed the presence of significant increase in cholesterol, triglycerides, glycaemia and total bilirubin. However, phosphatase alkaline and urea activities have been significantly decreased compared to the control group. No significant variation was observed for the rest of the studied parameters (high-density lipoproteins; low-density lipoproteins and uric acid). Additionally, multiple alterations, including cellular necrosis, leucocyte infiltration and congestion, were observed in different tissues of mice exposed to the tested HE.

Monitoring of Environmental Hg Occurrence in Tunisian Coastal Areas.

Total mercury (Hg) was determined in 450 environmental samples (seawater, sediment plant and fish) from five Mahdia coastal areas (Tunisia). Tolerable Weekly Intake% (TWI) values, according to the European Food Safety Authority (EFSA), were calculated based on the average metal concentration in fish and the average weekly fish consumption rate. Hg was accumulated mainly in fish and in Posidonia oceanica leaves. Hg in sediment ranged from 1.88 µg/kg dry weight (d.w.) to 7.48 µg/kg d.w., while it was between 0.32 µg/kg and 0.19 µg/kg in seawaters. Our study showed high concentration in Posidonia oceanica in S3 (plant = 16.76 ± 4.48 µg/kg d.w.) as compared to those in S4 sites (plant = 5.33 ± 0.05 µg/kg d.w.). Concentrations for S. aurata and S. salpa in the Rejiche area exceeded the EC 1881/2006 legislation with values of 1.9 mg/kg and 2.5 mg/kg, respectively, and consumers may be exposed to high concentrations of Hg that exceeds the EFSA. The results showed that the fish species should be constantly monitored due to their TWI% of 154.5% for S. aurata and 209.8% S. salpa respectively.

Short-time irrigation on young olive tree (Olea europaea L. cv. Chemlali) with untreated industrial poultry wastewater: investigation of growth parameters and leaves chemical composition.

The purpose of this study was to evaluate the short-term irrigation effect with industrial poultry wastewater on young olive trees (Olea europaea L. cv. Chemlali). Industrial poultry wastewater can be considered as a bio-fertilizer due to its richness in nutritive elements (SO42-, HCO3-, total nitrogen, and K+). The physicochemical analysis of wastewater showed a high concentration of TSS, COD, BOD, COT, NO3-, and conductivity. Measurements indicated that poultry wastewater enhanced plant growth, leaves dry matter, and ashes in comparison with tap water, as well as poultry wastewater diluted with tap water; however, a decrease in total soluble sugars (glucose and fructose) was detected in leaves. The determination of fatty acid profile of young olive trees leaves irrigated with poultry wastewater showed richness on saturated fatty acids in comparison with mono- and poly-unsaturated ones. In addition, oleic acid (C18:1) presented the lowest content in leaves of trees irrigated with poultry wastewater irrigation. According to those results, poultry wastewater lends itself to being a hydric alternative and at the same time a source of nutrients that can help fill the water deficit in semi-arid countries and avoid costly waste disposal for slaughterhouses.

Identification and quantification of plasticizers, bisphenol, and environmental toxic mineral elements residues in medicines from Tunisian markets.

In this study, 18 plasticizer (phthalates, adipates, sebacates, and others) residues in sixteen medicines available in Tunisian pharmaceutical markets are evaluated by MSPD combined with GC-MS. In parallel, in this research, UPLC-MS/MS technique was performed to detect bisphenol A, and cadmium, lead, chromium, cobalt, nickel, mercury, selenium, and arsenic levels were determined by ICP-MS. The maximum daily ingested mass of mineral elements was calculated and compared with permissible daily exposure limits published by USP NF 2013. Our results demonstrated that the 50% of drugs contained similar concentrations of di-ethyl adipate. Di-isobutyl phthalate was found in a single sample with a high concentration (1.07mgkg-1). Di-methyladipate was also present in only one sample. Diethyl phthalate was detected in three medicines: the highest concentration (17.03mgkg-1) was found in capsules. The other plasticizers and bisphenol A levels were below the limits of quantification in the all samples. All tested products were safe for use, except two clay-originated products. Concentrations and the maximum daily ingested mass of lead and arsenic were higher than recommended limits. The use of these medicines should require more attention taking into account the serious hazards of heavy metals to human health.

Persistent organic and inorganic pollutants in the effluents from the textile dyeing industries: Ecotoxicology appraisal via a battery of biotests.

Today, the textile industry is considered as a leading economic sector in Tunisia. However, this sector demands a huge volume of water and a wide spectrum of chemicals, which is converted into potentially toxic wastewater leading to environmental perturbation and human health toxicity. Assessment of the environmental risks associated with textile wastewater becomes a necessity. In this study, textile dyeing wastewater samples were collected before and after the physico-chemical treatment carried out by textile companies located in Monastir-city-Tunisia and subjected to chemical analyzes in order to determine their physicochemical characteristics and the content of metals and textile dyes. The ecotoxicological assessment was performed using four organisms, namely Selenastrum capricornutum, Vibrio fischeri, Daphnia magna and Lepidium sativum, to represent different trophic levels. Based on chemical data, some physicochemical parameters (e.g. TSS, COD and TSS levels) and metals (e.g. Cr, Hg and Sb) in the textile dyeing effluents were revealed not in compliance with the Tunisian standard. Moreover, high quantities of three disperse dyes have been detected even in the textile dyeing wastewater samples before and after treatments. The ecotoxicological data confirmed that the textile dyeing influents displayed toxic effects to all the test organisms, with Selenastrum capricornutum being the most sensitive organism. While, the above toxic effects were decreased slightly when evaluating the treated effluents. Metals and textile disperse dyes could be associated with the observed toxic effects of the textile influents and effluents. In fact, the treatment process applied by the evaluated companies was only partially efficient at removing metals, disperse dyes and effluent ecotoxicity, suggesting potential risks to aquatic biota. These findings emphasize the importance of applying integrated chemical and biological approaches for continuous evaluation of the toxicity of the treated effluents to predict hazards on the environment.

Evaluating the effect of dermaseptin S4 and its derivatives on multidrug-resistant bacterial strains and on the colon cancer cell line SW620.

Dermaseptins are peptides found in the skin secretions of Phyllomedusinae frogs. These peptides exert a lytic action on various microorganisms and have no considerable hemolytic effect except dermaseptin S4 (DS4) which exhibits a powerful cytotoxic effect. Therefore, we synthesized several analogs of DS4 in an attempt to find molecules with a weak hemolytic effect and significant bioactivities. In this study, we performed the synthesis of truncated peptides by introducing C-terminal and N-terminal amino acid deletions of the native sequence. All peptide analogs, in comparison with parental peptide, were tested firstly on human red blood cells to work out their cytotoxicity, secondly on the multidrug-resistant bacteria by trying to find MICs, and finally on colon cancer tumor cell line SW620 using the MTT test so as to investigate the anti-proliferative effect. Our results showed that, on the one hand, the N terminus of the native peptide was necessary for the antibacterial activity and the anti-proliferative effect of the peptide. On the other hand, the hemolytic activity was more notable in the sequences broken down on the C-terminal side.

Phthalates and non-phthalate plasticizers in Tunisian marine samples: Occurrence, spatial distribution and seasonal variation.

Seawater, sediment, seagrass and fish from several sites along the Tunisian coast were monitored for several phthalate esters (PAEs) and non-phthalate plasticizers (NPPs) during 2018-2019. In water and sediment, NPPs were higher than PAEs, being di(2-ethylhexyl) phthalate (DEHP, 0.0717 and 4.59 µg/g), and di(2-ethylhexyl) terephthalate (DEHT, 0.634 and 2.42 µg/g) most abundant. As expected, sediments acted as a sink for plasticizers, thus revealing a stronger contamination than water. Seagrass was less contaminated than fish, being DEHP (0.726 and 1.77 µg/g) and DEHT (9.19 and 23.2 µg/g) predominant. Biota poorly concentrated/accumulated plasticizers from water and sediment depending on the logKoct/wat and water solubility of single congeners. The spatial distribution of plasticizers was affected by the proximity to anthropogenic sources and the rate of coastal currents; whereas their seasonal variation may be related to the length of time of touristic/industrial inputs and factors such as rainfall and urban stormwater runoff.

Photocatalytic and biodegradation treatments of paracetamol: investigation of the in vivo toxicity.

Medicines and drugs consumption by all populations of the world can be expected to result in the contamination of the environment since 30-90% of residual drugs will be found into wastewaters. In this study, we investigate the degradation of acetaminophen, selected as a xenobiotic model molecule, via two separate procedures, the TiO2 impregnated on cellulosic paper photocatalysis, and specific bacterial biodegradation process. Results showed that for initial drug content of 400 mg/L and after 5 hours of processing, around 85% of paracetamol was photocatalytically degraded. The use of Pseudomonas putida E1.21 isolate allowed an abatement of around 92% after 32 h of processing. The acetaminophen toxicity conducted in vivo on laboratory mice showed a net decrease of the creatinine release and enzymes activities like ALP, ALT, AST, and LDH decreased significantly (p < 0.05) when mice were treated distinctly by acetaminophen treated with UV/TiO2 and the Pseudomonas putida E1.21 strain compared with the control experiments. CAT, MDA, and AchE serum level disruption measurement indicated a serious affection of the mice antioxidant system. These results were found to be in correlation with the ones of the histological analysis of the liver and kidney.

Ecotoxicity profile of heavily contaminated surface water of two rivers in Tunisia.

Persistent organic and inorganic contaminants generated by industrial effluent wastes poses a threat to the maintenance of aquatic ecosystems and public health. The Khniss and Hamdoun rivers, located in the central-east of Tunisia, receive regularly domestic and textile wastewater load. The present study aimed to survey the water quality of these rivers using physicochemical, analytical and toxicological approaches. In the physicochemical analysis, the recorded levels of COD and TSS in both samples exceed the Tunisian standards. Using the analytical approach, several metals and some textile dyes were detected. Indeed, 17 metals were detected in both samples in varying concentrations, which do not exceed the Tunisian standards. The sources of metals pollution can be of natural and anthropogenic origin. Three textile disperse dyes were detected with high levels compared to other studies: the disperse orange 37 was detected in the Khniss river with a concentration of 6.438 µg/L and the disperse red 1 and the disperse yellow 3 were detected in the Hamdoun river with concentrations of 3.873 µg/L and 1895 µg/L, respectively. Textile activities were the major sources of disperse dyes. For both samples, acute and chronic ecotoxicity was observed in all the studied organisms, however, no genotoxic activity was detected. The presence of metals and textile disperse dyes could be associated with the ecotoxicological effects observed in the river waters, in particular due to the industrial activity, a fact that could deteriorate the ecosystem and therefore threaten the human health of the population living in the study areas. Combining chemical and biological approaches, allowed the detection of water ecotoxicity in testing organisms and the identification of possible contributors to the toxicity observed in these multi-stressed water reservoirs.

Assessment of natural coagulants to remediate Tunisian textile wastewater by combining physicochemical, analytical, and toxicological data.

Due to the complexity and variability of textile wastewater composition, a constant search for new treatment strategies that are efficient, eco-friendly, and cost-effective is mandatory. In the present study, the efficiency of coagulation-flocculation using biocoagulants derived from cactus Opuntia ficus indica and eggplant Solanum melongena to remove toxic compounds from Tunisian textile wastewater samples was evaluated by combining assays to investigate physicochemical properties and in vitro (geno)toxicity with analytical chemistry. Both natural coagulants could significantly improve the physicochemical properties of the textile wastewater samples compared to the traditionally used chemical coagulant. The highest rate of decolorization was achieved after treatment with the cactus-derived coagulant. The analytical study revealed the presence of only crystal violet dye (CV) in only one sample. Both natural coagulants were able to remove CV, which may (partially) explain the decolorization of the treated samples. Only one untreated textile effluent induced a genotoxic response in the VITOTOX® assay. The genotoxic effect was not linked to the presence of CV and was no longer observed after treatment with each of the natural coagulants, suggesting the effectiveness of the remediation treatments to remove potentially genotoxic compound(s). However, in the other genotoxicity tests, no biologically relevant effects were observed for any of the tested samples. In conclusion, although the physicochemical data indicate that the use of natural coagulants (cactus and eggplant) could be an interesting alternative treatment process to the chemical coagulant for detoxifying textile effluents, these results were only partially supported by the toxicological and analytical data.

High leaf fluctuating asymmetry in two native plants growing in heavy metal-contaminated soil: the case of Metlaoui phosphate mining basin (Gafsa, Tunisia).

In order to estimate pollution impact in soil and plants by phosphates mining activities, three mining sites and one control site were selected around Metlaoui (phosphates basin of Gafsa, southern Tunisia) in March 2019. Heavy metal concentrations in soil and in two native plants (Moricandia arvensis and Diplotaxis harra: Brassicaceae) were measured using atomic absorption spectrometry. Development instability was estimated using leaf fluctuating asymmetry of the two plant species. The results showed that the soils of the Metlaoui sites contain higher levels of Cd, Ni, Zn, and Cr compared with control site (maximum of 33.225 ± 2.588; 100.86 ± 0.314; 180.267 ± 16.103; and 16.554 ± 0.313 ppm, respectively). These levels are higher than the maximal contents tolerated in soils. Heavy metals also occurred at high concentrations in plant organs in Metlaoui sites especially in leaves. Cadmium and Zn concentrations in these plants exceed the phytotoxic level (with maximum of 20.498 ± 0.627 ppm and maximum of 337.901 ± 16.686 ppm, respectively). Statistical analysis showed that fluctuating asymmetries (FA) of leaves of the two sampled plants are higher in mining sites compared with control. Pearson correlation and PCA analysis showed that FA is related to Cd, Ni, and Zn concentrations in soil. The results indicate that phosphate extraction and laundries in the mining sites caused heavy metal pollution in soil that accumulates in plant organs and caused also development instability. Our study showed that Moricandia arvensis may have the potential for phytoremediation.

Monitoring hospital wastewaters for their probable genotoxicity.

Hospitals' effluents contain a considerable amount of chemicals. Considering the significant volume of wastewater discharged by hospitals, the presence of these chemicals represents a real threat to the environment and human health. Thus, the aim of this study was to evaluate the in vivo and in vitro genotoxicities of three wastewater effluents collected from Tunisian hospitals. The liver of Swiss albino male mice, previously treated with different doses of the hospital wastewaters, was used as a model to detect DNA fragmentation. Our results showed all the hospital effluents caused significant qualitative and quantitative hazards in hepatic DNA. The wastewater collected from Sfax hospital exhibited the highest genotoxic effect, which may be explained by the presence in this effluent of some toxic micropolluants. There was a significant increase in genotoxicity, proportionally to the concentration of effluent. However, the vitotox assay did not show any significant genotoxicity on Salmonella typhimurium TA104 in the presence or absence of microsomal fraction S9. The ratio gentox/cytox was lower than the threshold 1.5. This study assessed the toxicological risk issued from Tunisian hospital wastewaters, which is potentially very harmful, and it has been pointed out that wastewater treatment requires special attention.

Optimization of polysaccharides extraction from quince peels: partial characterization, antioxidant and antiproliferative properties.

In this study, Box-Behnken Design was used to optimize the ultrasonic extraction of polysaccharides from quince peels (QPPs) by ascorbic acid and the effect of extraction temperature, extraction time and pH was evaluated. Under optimized conditions of temperature 90 °C, 60 min sonication time and pH = 3.26, the extraction yield, the galacturonic acid yield and the concentration of sample required to scavenge 50% of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) values of QPPs were respectively 10.25%, 3.86% and 1.35 mg/mL. The QPPs extracted under optimum conditions was characterized by Fourier transform infrared spectroscopy (FTIR), Nuclear magnetic resonance (1 H NMR) and Size exclusion chromatography (SEC/MALS/VD/DRI). The monosaccharide analysis revealed that arabinose was the most abundant, followed by galactose, glucose, mannose and xylose. Moreover, QPPs showed significant antioxidant activities (2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric- reducing antioxidant power (FRAP)) and reduced viability of human Caco-2 and murine B-16 cell lines in a dose-dependent manner. Hence QPPs could be used as antitumor agent in functional foods andpharmaceutical industries.

Effectiveness of dairy treated wastewater and different irrigation systems on the growth, biomass and fruiting of a Tunisian olive orchard (Olea europaea L., cv Chemlali).

The effectiveness of dairy treated wastewater (TWW) was evaluated, in comparison with tap water (TW), on a Tunisian olive orchard (Olea europea, cv Chemlali), irrigated manually (MI) and by surface dripping (SDI). To this purpose, tree growth, biomass and fruiting were monthly tested for a one-year period. Similar trunk diameters, nodes/tree, root lengths were obtained, independently of source and system of irrigation. Also, comparable tree length and leaf area, shoots/tree and biomass, were observed between TWW and TW plants. However, such parameters improved under SDI rather than MI. Fruiting occurred only in TWW and TW trees treated by MI. Concerning growth, biomass and fruiting, TWW represented a valid alternative source for the irrigation of olive orchard, especially in Tunisia, already facing the freshwater scarcity. Monitoring of soil, TWW, fruits and oil will be required to validate the use of such effluent for olive orchard irrigation.

Toxic effect of alpha cypermethrin, an environmental pollutant, on myocardial tissue in male wistar rats.

α-Cypermethrin (CYP) is a pyrethroid insecticide-like environmental pollutant, widely found in the environment. New research links exposure to high levels of CYP to health damage; however, little is known about the effect of CYP on cardiovascular disease. The purpose of the present study was to evaluate, for the first time, biochemical and cardiovascular changes in male rats resulting from subchronic CYP exposure. The animals were divided into three groups: group 1 served as the control, group 2 (CYP1) received 4 mg/kg of CYP by gavage, and group 3 (CYP2) received 8 mg/kg of CYP by gavage, for 8 weeks each. Results showed that both CYP1 and CYP2 markedly increased plasma concentrations of cardiac markers (LDH, CK-MB, and troponin-T). Moreover, compared to the control group, CYP treatment elevated cardiac oxidative stress, as shown by increased MDA level and decreased activity of SOD, CAT, and GSH-Px. In addition, CYP2 caused a significant increase of 42% the concentration of total cholesterol and more than 75% in triglycerides compared to the control group. Furthermore, DNA fragmentation and collagen deposition were both amplified owing to CYP toxicity. This harmful effect was confirmed by a histological study using H-E and Sirius Red staining. Overall, our results clearly proved the cardiotoxicity caused by α-cypermethrin.